超分子自組裝之可逆網狀交聯高分子於細胞貼附之應用

Abstract

近幾十年來，具備生物可相容性與生物可分解性的生物醫學材料在醫學工程和組織修復領域上的應用逐漸受到重視。直鏈型的聚酯類 高分子尤其是眾多生物醫學材料中被廣泛應用的一類。聚己內酯具有高結晶性和高親脂性，降解所需耗費時間的時間較長，因此在本研究中，以共聚物(Copolymer)為主體，結合非共價鍵結中之多點式氫鍵作用力化合物形成一個網狀交聯結構之材料，以提高作為細胞貼附系統之性質。進行分析後，具有網狀交聯結構的高分子材料展現絕佳熱穩定度、機械強度、生物相容性以及有效地促進細胞的貼附能力。另一方面，在細胞培養的實驗中，其表面細胞之生長密度與商業品NunclonTM∆ 幾乎相近，證明利用氫鍵作用力改質過後的高分子材料極具發展潛力做為新世代組織工程的支架。

In the last several decades, biomaterials which possess both biocompatibility and biodegradability are gaining popularity in tissue engineering applications. Aliphatic polyesters are especially being used in these fields for applications. Poly(ε-caprolactone) (PCL) exhibits certain desirable characteristics for biomaterial applications. However, high crystallinity and hydrophobicity result in its long degradation time. Therefore, a new concept to modify and enhance properties of existed functional PCL through self-constituted multiple hydrogen bonding (SCMHB) interactions has been exploited. In this study, a new SCMHB-grafted polymer has been prepared which exhibits high thermal stability, mechanical property, excellent biocompatibility and the ability to promote cell adhesion in the solid state owing to intermolecular hydrogen bonds induced physical cross-linking. In addition, they also can promote cell attachment by investigation of cell culture, a remarkable improvement in performance relative to a conventional NunclonTM® under similar experimental conditions has been achieved. Thus, these results illustrate the opportunity to provide the next generation of tissue engineering scaffolds.