標題: MrkF is a component of type 3 fimbriae in Klebsiella pneumoniae
作者: Huang, Ying-Jung
Liao, Hsin-Wei
Wu, Chien-Chen
Peng, Hwei-Ling
生物科技學系
Department of Biological Science and Technology
關鍵字: Type 3 fimbriae;MrkF;Klebsiella pneumoniae
公開日期: 1-Jan-2009
摘要: Klebsiella pneumoniae type 3 fimbriae are encoded by mrkABCDF genes which produce the major pilin subunit MrkA, chaperone MrkB, outer membrane usher MrkC, adhesin MrkD and MrkF of unknown function, respectively. RT-PCR analysis demonstrated that the mrkF gene is contained within the mrk operon. Deletion of mrkF in K. pneumoniae CG43 was found to reduce biofilm formation. A higher level of biofilm formation activity was also observed in recombinant Escherichia coli JM109[pmrkABCDF] compared to that observed for JM109[pmrkABCD]. Immunoelectron microscopy analysis of recombinant type 3 fimbriae using anti-MrkA and anti-MrkF antibody-labeled gold particles revealed that MrkF intermittently inserted into the MrkA filament. An interaction between recombinant MrkA and MrkF was demonstrated by co-immunoprecipitation analysis, further supporting the notion that MrkF is a structural component of the fimbriae. Intriguingly, the incorporation of MrkF appeared to decrease fimbrial length but increased activity of autoaggregation and biofilm formation in the bacteria JM109[pmrkABCDF]. This suggested that MrkF may play a role in assembly of the filament. (C) 2008 Elsevier Masson SAS. All rights reserved.
URI: http://dx.doi.org/10.1016/j.resmic.2008.10.009
http://hdl.handle.net/11536/7875
ISSN: 0923-2508
DOI: 10.1016/j.resmic.2008.10.009
期刊: RESEARCH IN MICROBIOLOGY
Volume: 160
Issue: 1
起始頁: 71
結束頁: 79
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