藉由主動控制流體自動形成脂質雙層膜之特性研究

Abstract

膜蛋白的研究對於細胞運作上扮演相當重要的角色，而平面脂質雙層膜則廣泛地應用在膜蛋白的電生理研究，因此本研究中我們設計三種平面脂質雙層膜的成膜方式：painting method、脂質水溶液液滴成膜以及脂質體絕緣介電泳成膜來形成脂質雙層膜，因此我們成功地利用人工操作的方式在孔洞中形成平面脂質雙層膜，並且在光學上觀測到脂質雙層膜薄化以及黑色光圈我們稱為Plateau–Gibbs border，在電性量測上也可測得厚度約2-6 nm(電容值為：1 □F/cm2)，最後脂質水溶液液滴成膜以及脂質體絕緣介電泳成膜兩種方式可直接與介電濕潤系統整合來達成自動化晶片，在介電濕潤實驗方面我們成功利用共平面電極-介電濕潤技術來驅動目標的脂質水溶液，而絕緣結構介電泳的部分則可以操控DNA以及螢光粒子，未來將應用在脂質體上，而本研究的最終目的是希望可以達到微液滴自動化成膜晶片，並且與膜蛋白結合，成為光學-電性量測同步的單一離子通道生物感測晶片。

Membrane proteins play essential roles in cellular processes. A planar lipid bilayer is widely used for the electrophysiological study of membrane proteins. In this research, we fabricated EWOD-based platform with 50-150□□m aperture for bilayer lipid membrane (BLM) formation. And we design three types of BLM formation: painting method, lipid droplet-based formation and insulator-based (iDEP) of liposomes. We successfully yield planar lipid bilayer of about 2-6 nm thickness (1 □F/cm2) which is observed thinning and Plateau–Gibbs border situation on micro-pore chips in optical and electrical measurement. However, only lipid droplet-based formation and iDEP of liposomes formation could integrate electrowetting-on-dielectric (EWOD) system to be suited for automated processing. Co-planar EWOD has been used to transport potassium chloride droplets and lipid droplets. 3D iDEP has been demonstrated to trap DNA molecules and fluorescent beads. Eventually, we hope to achieve an automatic biochip for BLM formation and an optical–electrical single ion-channel sensor in the future on the basis of integration of the techniques investigated in this research.