完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Sheu, J. -T. | en_US |
dc.contributor.author | Chen, C. C. | en_US |
dc.contributor.author | Chang, K. S. | en_US |
dc.contributor.author | Li, Y. -K. | en_US |
dc.date.accessioned | 2014-12-08T15:11:11Z | - |
dc.date.available | 2014-12-08T15:11:11Z | - |
dc.date.issued | 2008-07-15 | en_US |
dc.identifier.issn | 0956-5663 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1016/j.bios.2008.02.024 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/8576 | - |
dc.description.abstract | Ultra-thin body of p-type field-effect transistors were developed as transducer for biosensors. Changes of conductance resulted from the changes of the surface potentials of ultra-thin body field-effect transistors (UTB-FETs) due to surface chemical modifications were demonstrated. The channel surface of UTB-FETs were modified with N-[3-(trimethoxysilyl)propyl]ethylenediamine (AEAPTMS) and then gold nanoparticles (AuNPs) to immobilize the bio-component, the genetically engineered Delta(5)-3-ketosteroid isomerase (Art_KSI) or the Art_KSI conjugated with charged reporter (Art_KSI_mA51). The binding of charge-based molecules or nanoparticles has been demonstrated to strongly affect the conductivity of UTB-FETs: the increase or decrease of the conductance depends on the polarity of the immobilized molecules or nanoparticles. A new protocol involving the detection of a non-charged analyte relied on the competitive binding of analyte (19-norandrostendione) and a charged reporter (mA51) with KSL When exposed to a 19-norandrostendione solution (10 mu M), the conductance of Art_KSI_mA51-modified UTB-FET increased by 265 nS (similar to 12%). On the other hand, conductance of Art_KSI_modified UTB-FET showed no distinct change under the same detection conditions. (c) 2008 Elsevier B.V. All rights reserved | en_US |
dc.language.iso | en_US | en_US |
dc.subject | biosensors | en_US |
dc.subject | ultra-thin body field-effect transistor | en_US |
dc.subject | N-[3(trimethoxysilyl)propyl]ethylenediamine | en_US |
dc.subject | gold nanoparticles | en_US |
dc.subject | Delta(5)-3-ketosteroid isomerase | en_US |
dc.subject | 19-norandrostendione | en_US |
dc.title | A possibility of detection of the non-charge based analytes using ultra-thin body field-effect transistors | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.bios.2008.02.024 | en_US |
dc.identifier.journal | BIOSENSORS & BIOELECTRONICS | en_US |
dc.citation.volume | 23 | en_US |
dc.citation.issue | 12 | en_US |
dc.citation.spage | 1883 | en_US |
dc.citation.epage | 1886 | en_US |
dc.contributor.department | 材料科學與工程學系奈米科技碩博班 | zh_TW |
dc.contributor.department | 應用化學系 | zh_TW |
dc.contributor.department | Graduate Program of Nanotechnology , Department of Materials Science and Engineering | en_US |
dc.contributor.department | Department of Applied Chemistry | en_US |
dc.identifier.wosnumber | WOS:000256736300021 | - |
dc.citation.woscount | 7 | - |
顯示於類別: | 期刊論文 |