標題: | Evidence of DNA transfer through F-pilus channels during Escherichia coli conjugation |
作者: | Shu, An-Chi Wu, Chien-Chen Chen, Yi-Yang Peng, Hwei-Ling Chang, Hwan-You Yew, Tri-Rung 生物科技學系 Department of Biological Science and Technology |
公開日期: | 1-七月-2008 |
摘要: | The mechanism of DNA transfer from Escherichia coli (E. coli) Hfr donor strain AT2453 to recipient strain AB1157 during the conjugation process has been investigated by liquid atomic force microscopy (AFM). With the success of immobilizing both E. coli strains on gelatin-treated glass under aqueous solution, the F-pilus between an E. coli mating pair could be clearly imaged and dissected by an AFM probe. Another AFM probe functionalized with an anti-single-stranded DNA (ssDNA) antibody was then applied to detect transferring ssDNA. According to the AFM force spectrum, the transferring ssDNA could be detected only in the dissected area with a binding force of 109 5 pN measured. Our results provide direct evidence indicating that the DNA was transferred through the F-pilus channel between an E. coli mating pair during their conjugation. |
URI: | http://dx.doi.org/10.1021/la703384n http://hdl.handle.net/11536/8653 |
ISSN: | 0743-7463 |
DOI: | 10.1021/la703384n |
期刊: | LANGMUIR |
Volume: | 24 |
Issue: | 13 |
起始頁: | 6796 |
結束頁: | 6802 |
顯示於類別: | 期刊論文 |