標題: Evidence of DNA transfer through F-pilus channels during Escherichia coli conjugation
作者: Shu, An-Chi
Wu, Chien-Chen
Chen, Yi-Yang
Peng, Hwei-Ling
Chang, Hwan-You
Yew, Tri-Rung
生物科技學系
Department of Biological Science and Technology
公開日期: 1-Jul-2008
摘要: The mechanism of DNA transfer from Escherichia coli (E. coli) Hfr donor strain AT2453 to recipient strain AB1157 during the conjugation process has been investigated by liquid atomic force microscopy (AFM). With the success of immobilizing both E. coli strains on gelatin-treated glass under aqueous solution, the F-pilus between an E. coli mating pair could be clearly imaged and dissected by an AFM probe. Another AFM probe functionalized with an anti-single-stranded DNA (ssDNA) antibody was then applied to detect transferring ssDNA. According to the AFM force spectrum, the transferring ssDNA could be detected only in the dissected area with a binding force of 109 5 pN measured. Our results provide direct evidence indicating that the DNA was transferred through the F-pilus channel between an E. coli mating pair during their conjugation.
URI: http://dx.doi.org/10.1021/la703384n
http://hdl.handle.net/11536/8653
ISSN: 0743-7463
DOI: 10.1021/la703384n
期刊: LANGMUIR
Volume: 24
Issue: 13
起始頁: 6796
結束頁: 6802
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