標題: | 1064 nm Deep Near-Infrared (NIR) Excited Raman Microspectroscopy for Studying Photolabile Organisms |
作者: | Ando, Masahiro Sugiura, Miwa Hayashi, Hidenori Hamaguchi, Hiro-o 應用化學系 Department of Applied Chemistry |
關鍵字: | Near-infrared Raman spectroscopy;Multichannel Raman spectroscopy;1064 nm excitation;Microspectroscopy;Cyanobacteria;Thermosynechococcus elongatus |
公開日期: | 1-五月-2011 |
摘要: | We have constructed a 1064 nm deep near-infrared (NIR) excited multichannel Raman microspectrometer using an InP/InGaAsP multichannel detector. This microspectrometer achieves high sensitivity suitable for in vivo measurements of single living cells with lateral resolution of 0.7 mu m and depth resolution of 3.1 mu m. It has been applied to the structural analysis of living cyanobacterial cells, well-known model organisms for photosynthesis research, which are too photolabile to be measured with visible laser excitation. High signal-to-noise ratio (SIN) Raman spectra have been obtained from carotenoid, chlorophyll a, and phycocyanin in a single living cyanobacterial cell with no appreciable interference from autofluorescence or photodamage. Sub-micrometer mapping of Raman intensities provides clear distribution images of the three pigments inside the cell. |
URI: | http://dx.doi.org/10.1366/10-06196 http://hdl.handle.net/11536/8958 |
ISSN: | 0003-7028 |
DOI: | 10.1366/10-06196 |
期刊: | APPLIED SPECTROSCOPY |
Volume: | 65 |
Issue: | 5 |
起始頁: | 488 |
結束頁: | 492 |
顯示於類別: | 期刊論文 |