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dc.contributor.authorWang, Ya-Jeanen_US
dc.contributor.authorPan, Kao-Luen_US
dc.contributor.authorHsieh, Tsung-Chihen_US
dc.contributor.authorChang, Teng-Yuanen_US
dc.contributor.authorLin, Wen-Hsingen_US
dc.contributor.authorHsu, John T. -A.en_US
dc.date.accessioned2014-12-08T15:11:47Z-
dc.date.available2014-12-08T15:11:47Z-
dc.date.issued2011-04-01en_US
dc.identifier.issn0163-3864en_US
dc.identifier.urihttp://dx.doi.org/10.1021/np100578uen_US
dc.identifier.urihttp://hdl.handle.net/11536/9033-
dc.description.abstractDiosgenirr (3 beta-hydroxy-5-spirostene, 1); a plant-derived sapogenin, is used as a dietary: supplement However, the biological effects of 1 related to viral replication remain unexplored. In this study, the effects of 1 on hepatitis C virus (HCV) replication were evaluated. Based on a reporter-based HCV subgenomic replicon system, 1 was found to inhibit HCV replication at low micromolar concentrations. The EC(50) (concentration at which 50% of HCV replication is inhibited) of 1 was 3.8 mu M No cellular toxicity was observed at this concentration.,Diosgenin, (1) also significantly reduced the levels of viral,RNA and viral proteins as evaluated by quantitative real-time reverse :transcriptase PCR and Western blot analysis, respectively. In addition, in an HCV antiviral system more closely aligned to all steps involved in the HCV infection and life cycle, 1 totally abolished HCV replication at 20 mu M. Moreover, 1 reduced the phosphorylation of signal transducer and activator of transcription 3. A combination of 1:and interferon-alpha exerted an additive effect on the resultant anti-HCV activity.en_US
dc.language.isoen_USen_US
dc.titleDiosgenin, a Plant-Derived Sapogenin, Exhibits Antiviral Activity in Vitro against Hepatitis C Virusen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/np100578uen_US
dc.identifier.journalJOURNAL OF NATURAL PRODUCTSen_US
dc.citation.volume74en_US
dc.citation.issue4en_US
dc.citation.spage580en_US
dc.citation.epage584en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000289742300006-
dc.citation.woscount6-
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