標題: Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H(2)O(2) generation from polyunsaturated fatty acids
作者: Cheng, Tsai-Mu
Mao, Simon J. T.
Lai, Shiau-Ting
Chang, Chia-Ching
Yang, Ming-Chi
Chen, Nai-Chi
Chou, Shiu-Ching
Pan, Ju-Pin
生物科技學院
College of Biological Science and Technology
公開日期: 1-Mar-2011
摘要: Patients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H(2)O(2) generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN(3), a peroxidase inhibitor, catalase, a H(2)O(2) decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H(2)O(2) production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN(3) and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression.
URI: http://dx.doi.org/10.3109/10715762.2010.532492
http://hdl.handle.net/11536/9260
ISSN: 1071-5762
DOI: 10.3109/10715762.2010.532492
期刊: FREE RADICAL RESEARCH
Volume: 45
Issue: 3
起始頁: 303
結束頁: 316
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