標題: Sub-diffraction-limit imaging using mode multiplexing
作者: Wang, Nan
Miyazaki, Jun
He, Jinping
Seto, Keisuke
Kobayashi, Takayoshi
電子物理學系
Department of Electrophysics
關鍵字: Confocal microscopy;Mode multiplexing;Superresolution;Image processing
公開日期: 15-May-2015
摘要: Pixel-by-pixel processed fluorescence difference microscopy is experimentally demonstrated by multiplexing excitation laser beams with Gaussian and donut spot shapes and then demultiplexing the fluorescent signals using lock-in amplifiers. With this scheme, a fixed sample of fluorescent spheres and a slice of mouse brain tissue are imaged with resolutions that exceed the diffraction limit. Compared to previously reported subtraction imaging techniques, this pixel-by-pixel scan can be applied to improve the resolution of a moving sample without introducing subtraction errors. The synchronized signal detection feature makes this method extendible to various applications. (C) 2014 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.optcom.2014.12.072
http://hdl.handle.net/11536/124442
ISSN: 0030-4018
DOI: 10.1016/j.optcom.2014.12.072
期刊: OPTICS COMMUNICATIONS
Volume: 343
起始頁: 28
結束頁: 33
Appears in Collections:Articles