标题: | 以香豆素之衍生物作為高選擇性硫醇及銅 (II) 離子螢光化學感測分子之合成與應用 Highly Selective Flourorescent Sensors for Thiols and Cu (II) ion Based on Coumarin and Their Applications in Living Cells |
作者: | 魏翎芳 Wei, Ling-fang 吳淑褓 Wu, Shu-Pao 應用化學系碩博士班 |
关键字: | 化學感測分子;硫醇;銅離子;香豆素;chemosensors;Thiols;Copper ion;Coumarin |
公开日期: | 2015 |
摘要: | 本論文以coumarin為螢光基團,設計合成螢光化學感測分子AC和 PHC。 化合物AC藉由藍色螢光增強方法來辨識硫醇,即使在有其他胺基 酸存在的環境中,仍對cysteine和homocysteine有高度的選擇性。AC在 緩衝溶液中都具有良好的偵測能力,對於cysteine和homocysteine偵測 極限分別為 65 和 78 nM,而且,在pH值6.0到9.0時,有較明顯的偵 測效果。以HPLC分離並送測質譜,證明AC反應成7-hydroxycoumarin, 而產生藍色螢光。並進一步應用於 RAW 264.7 cells 內偵測硫醇之存 在。 PHC藉由藍色螢光增強方法來辨識銅離子,在有其他金屬離子存 在的環境中,對銅離子有高度的選擇性。PHC在緩衝溶液中具有良好 的偵測能力,對於銅離子偵測極限為 8 nM。並進一步應用於 RAW 264.7 cells 內偵測銅離子之存在。 In this thesis, coumarin was used as a signal unit for chemosensors AC and PHC. Chemosensor AC indicates the presence of cysteine and homocysteine among other amino acids with high selectivity by a significant blue emission. Chemosensor AC displays good sensitivity in buffer solution at the pH value 6.0 to 9.0. The detection limits of AC for cysteine and homocysteine are 65 and 78 nM, respectively. In addition, chemosensor AC can be used as a fluorescent probe for detecting cysteine and homocysteine in living cells. Chemosensor PHC indicates the presence of copper ions among other amino acids with high selectivity by a significant blue emission. The detection limit of PHC for Cu (II) is 8 nM. In addition, chemosensor PHC can be used as a fluorescent probe for detecting Cu (II) in living cells. |
URI: | http://140.113.39.130/cdrfb3/record/nctu/#GT070252535 http://hdl.handle.net/11536/126547 |
显示于类别: | Thesis |