標題: A highly selective turn-on fluorescent probe for hypochlorous acid based on hypochlorous acid-induced oxidative intramolecular cyclization of boron dipyrromethene-hydrazone
作者: Chen, Wei-Chieh
Venkatesan, Parthiban
Wu, Shu-Pao
應用化學系
Department of Applied Chemistry
關鍵字: Hypochlorous acid;Fluorescent probe;Boron dipyrromethene;Bioimaging
公開日期: 2-Jul-2015
摘要: A BODIPY-based fluorescent probe, HBP, was developed for the detection of hypochlorous acid based on the specific hypochlorous acid-promoted oxidative intramolecular cyclization of heterocyclic hydrazone in response to the amount of HOCl. The reaction is accompanied by a 41-fold increase in the fluorescent quantum yield (from 0.004 to 0.164). The fluorescence intensity of the reaction between HOCl and HBP is linear in the HOCl concentration range of 1-8 mu M with a detection limit of 2.4 nM (S/N = 3). Confocal fluorescence microscopy imaging using RAW264.7 cells showed that the new probe HBP could be used as an effective fluorescent probe for detecting HOCl in living cells. (C) 2015 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.aca.2015.04.012
http://hdl.handle.net/11536/127860
ISSN: 0003-2670
DOI: 10.1016/j.aca.2015.04.012
期刊: ANALYTICA CHIMICA ACTA
Volume: 882
起始頁: 68
結束頁: 75
Appears in Collections:Articles