標題: Plasmon-induced photoelectrochemical biosensor for in situ real-time measurement of biotin-streptavidin binding kinetics under visible light irradiation
作者: Guo, Jingchun
Oshikiri, Tomoya
Ueno, Kosei
Shi, Xu
Misawa, Hiroaki
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Photoelectrochemical biosensor;Localized surface plasmon;Kinetic analysis;Visible light-response
公開日期: 8-Mar-2017
摘要: We developed a localized surface plasmon-induced visible light-responsive photoelectrochemical (PEC) biosensor using a titanium dioxide (TiO2) photoelectrode loaded with gold nanoislands (AuNIs) for in situ real-time measurement of biotin-streptavidin association. As a proof of concept, self-assembled thiol-terminated biotin molecules bound on a AuNls/TiO2 photoelectrode were successfully utilized to explore the photocurrent response to streptavidin-modified gold nanoparticle (STA-AuNP) solutions. This plasmon-induced PEC biosensor is simple and easy to miniaturize. Additionally, the PEC biosensor achieves highly sensitive measurements under only visible light irradiation and prevents the UV-induced damage of samples. Furthermore, a novel approach has been proposed to realize the real-time monitoring of biotin-STA binding affinities and kinetics by analyzing the PEC sensing characteristics. This PEC biosensor and novel analysis method could provide a new approach for the specific electrical detection and real-time kinetic measurements for clinical diagnostics and drug development. (C) 2016 The Authors. Published by Elsevier B.V.
URI: http://dx.doi.org/10.1016/j.aca.2016.12.025
http://hdl.handle.net/11536/133137
ISSN: 0003-2670
DOI: 10.1016/j.aca.2016.12.025
期刊: ANALYTICA CHIMICA ACTA
Volume: 957
起始頁: 70
結束頁: 75
Appears in Collections:Articles