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dc.contributor.authorWu, Jennifer Yun-Shinen_US
dc.contributor.authorLin, Chih-Hengen_US
dc.contributor.authorFeng, Mei-Hueien_US
dc.contributor.authorChen, Chien-Hungen_US
dc.contributor.authorSu, Ping-Chiaen_US
dc.contributor.authorYang, Po-Wenen_US
dc.contributor.authorZheng, Jian-Mingen_US
dc.contributor.authorFu, Chang-Weien_US
dc.contributor.authorYang, Yuh-Shyongen_US
dc.date.accessioned2017-04-21T06:55:45Z-
dc.date.available2017-04-21T06:55:45Z-
dc.date.issued2016-04en_US
dc.identifier.issn1940-087Xen_US
dc.identifier.urihttp://dx.doi.org/10.3791/53660en_US
dc.identifier.urihttp://hdl.handle.net/11536/134155-
dc.description.abstractSurveillance using biomarkers is critical for the early detection, rapid intervention, and reduction in the incidence of diseases. In this study, we describe the preparation of polycrystalline silicon nanowire field-effect transistors (pSNWFETs) that serve as biosensing devices for biomarker detection. A protocol for chemical and biomolecular sensing by using pSNWFETs is presented. The pSNWFET device was demonstrated to be a promising transducer for real-time, label-free, and ultra-high-sensitivity biosensing applications. The source/drain channel conductivity of a pSNWFET is sensitive to changes in the environment around its silicon nanowire (SNW) surface. Thus, by immobilizing probes on the SNW surface, the pSNWFET can be used to detect various biotargets ranging from small molecules (dopamine) to macromolecules (DNA and proteins). Immobilizing a bioprobe on the SNW surface, which is a multistep procedure, is vital for determining the specificity of the biosensor. It is essential that every step of the immobilization procedure is correctly performed. We verified surface modifications by directly observing the shift in the electric properties of the pSNWFET following each modification step. Additionally, X-ray photoelectron spectroscopy was used to examine the surface composition following each modification. Finally, we demonstrated DNA sensing on the pSNWFET. This protocol provides step-by-step procedures for verifying bioprobe immobilization and subsequent DNA biosensing application.en_US
dc.language.isoen_USen_US
dc.subjectBioengineeringen_US
dc.subjectIssue 110en_US
dc.subjectPolysiliconen_US
dc.subjectnanowire field-effect transistoren_US
dc.subjectbiosensingen_US
dc.subjectsurface modificationen_US
dc.subjectcharge-charge interactionen_US
dc.subjectlabel-freeen_US
dc.subjectreal-time detectionen_US
dc.titlePreparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applicationsen_US
dc.identifier.doi10.3791/53660en_US
dc.identifier.journalJOVE-JOURNAL OF VISUALIZED EXPERIMENTSen_US
dc.citation.issue110en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.identifier.wosnumberWOS:000380256000031en_US
Appears in Collections:Articles