標題: Overcoming Multidrug Resistance of Breast Cancer Cells by the Micellar Drug Carriers of mPEG-PCL-graft-cellulose
作者: Chen, Yung-Tsung
Chen, Chao-Hsuan
Hsieh, Ming-Fa
Chan, Ann Shireen
Liau, Ian
Tai, Wan-Yu
應用化學系分子科學碩博班
Institute of Molecular science
關鍵字: methoxy-poly (ethylene glycol);poly (epsilon-caprolactone);multidrug resistance;P-glycoprotein
公開日期: 2009
摘要: The amphiphilic block copolymers methoxypoly (ethylene glycol)-poly (epsilon-caprolactone) (mPEG-PCL) was grafted to 2-hydroxyethyl cellulose (HEC) to produce water-soluble copolymers. Doxorubicin (DOX)-loaded nanoparticles were prepared by dialysis method and the sizes of nanoparticles were determined by dynamic light scattering (DLS) in solution. The size of the nanoparticles was in the range of 197.4 to 340.7 nm. Rhodamine 123 was used to probe the relative P-glycoprotein (P-gp) expression in human breast cancer cell lines MCF-7/WT and MCF-7/ADR. Confocal laser scanning microscopy (CLSM) showed a difference between the fluorescence images of DOX-loaded micelles and free DOX. For a quantitative basis, flow cytometry was done on both cell lines treated with DOX-loaded micelles and free DOX to compare a difference in effect. The amount of DOX-loaded micelles in MCF-7/ADR increased compared to that in the MCF-7/WT cells. This gives insight into how the micellar system developed in this study overcomes the multidrug resistance (MDR) effect.
URI: http://hdl.handle.net/11536/134900
ISBN: 978-3-540-92840-9
ISSN: 1680-0737
期刊: 13TH INTERNATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING, VOLS 1-3
Volume: 23
Issue: 1-3
起始頁: 1224
結束頁: +
Appears in Collections:Conferences Paper