標題: Depicting Binding-Mediated Translocation of HIV-1 Tat Peptides in Living Cells with Nanoscale Pens of Tat-Conjugated Quantum Dots
作者: Lin, Chien Y.
Huang, Jung Y.
Lo, Leu-Wei
田家炳光電中心
Tin Ka Ping Photonics Center
關鍵字: single-particle tracking;cell-penetrating peptides;heparan sulfate proteoglycan;actin filaments;live cell;plasma membrane
公開日期: 1-Feb-2017
摘要: Cell-penetrating peptides (CPPs) can translocate across cell membranes, and thus have great potential for the cellular delivery of macromolecular cargoes. However, the mechanism of this cellular uptake process is not yet fully understood. In this study, a time-lapse single-particle light-sheet microscopy technique was implemented to obtain a parallel visualization of the translocating process of individual human immunodeficiency virus 1 (HIV-1) transactivator of transcription (Tat) peptide conjugated quantum dots (TatP-QDs) in complex cellular terrains. Here, TatP-QDs served as nanoscale dynamic pens, which depict remarkable trajectory aggregates of TatP-QDs on the cell surface. Spectral-embedding analysis of the trajectory aggregates revealed a manifold formed by isotropic diffusion and a fraction of directed movement, possibly caused by interaction between the Tat peptides and heparan sulfate groups on the plasma membrane. Further analysis indicated that the membrane deformation induced by Tat-peptide attachment increased with the disruption of the actin framework in cytochalasin D (cyto D)-treated cells, yielding higher interactions on the TatP-QDs. In native cells, the Tat peptides can remodel the actin framework to reduce their interaction with the local membrane environment. Characteristic hot spots for interaction were detected on the membrane, suggesting that a funnel passage may have formed for the Tat-coated particles. This finding offers valuable insight into the cellular delivery of nanoscale cargo, suggesting an avenue for direct therapeutic delivery.
URI: http://dx.doi.org/10.3390/s17020315
http://hdl.handle.net/11536/144131
ISSN: 1424-8220
DOI: 10.3390/s17020315
期刊: SENSORS
Volume: 17
Issue: 2
起始頁: 0
結束頁: 0
Appears in Collections:Articles


Files in This Item:

  1. 3f896bd6ac39873f70d579478ba243ac.pdf

If it is a zip file, please download the file and unzip it, then open index.html in a browser to view the full text content.