標題: N-Doped Graphene Quantum Dots-Decorated V2O5 Nanosheet for Fluorescence Turn Off-On Detection of Cysteine
作者: Ganganboina, Akhilesh Babu
Chowdhury, Ankan Dutta
Doong, Ruey-An
環境工程研究所
Institute of Environmental Engineering
關鍵字: N-GQDs;V2O5 nanosheet;turn off-on sensing;cysteine detection;redox reaction;fluorescence
公開日期: 10-Jan-2018
摘要: The development of a fast-response sensing technique for detection of cysteine can provide an analytical platform for prescreening of disease. Herein, we have developed a fluorescence turn off-on fluorescence sensing platform by combining nitrogen-doped graphene quantum dots (N-GQDs) with V2O5 nanosheets for the sensitive and selective detection of cysteine in human serum samples. V2O5 nanosheets with 2-4 layers are successfully synthesized via a simple and scalable liquid exfoliation method and then deposited with 2-8 nm of N-GQDs as the fluorescence turn off-on nanoprobe for effective detection of cysteine in human serum samples. The V2O5 nanosheets serve as both fluorescence quencher and cysteine recognizer in the sensing platform. The fluorescence intensity of N-GQDs with quantum yield of 0.34 can be quenched after attachment onto V2O5 nanosheets. The addition of cysteine triggers the reduction of V2O5 to V4+ as well as the release of N-GQDs within 4 min, resulting in the recovery of fluorescence intensity for the turn off-on detection of cysteine. The sensing platform exhibits a two-stage linear response to cysteine in the concentration range of 0.1-15 and 15-125 mu M at pH 6.5, and the limit of detection is 50 nM. The fluorescence response of N-GQD@V2O5 exhibits high selectivity toward cysteine over other 22 electrolytes and biomolecules. Moreover, this promising platform is successfully applied in detection of cysteine in human serum samples with excellent recovery of (95 +/- 3.8) - (108 +/- 2.4)%. These results clearly demonstrate a newly developed redox reaction-based nanosensing platform using N-GQD@V2O5 nanocomposites as the sensing probe for cysteine-associated disease monitoring and diagnosis in biomedical applications, which can open an avenue for the development of high performance and robust sensing probes to detect organic metabolites.
URI: http://dx.doi.org/10.1021/acsami.7b15120
http://hdl.handle.net/11536/144383
ISSN: 1944-8244
DOI: 10.1021/acsami.7b15120
期刊: ACS APPLIED MATERIALS & INTERFACES
Volume: 10
起始頁: 614
結束頁: 624
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