標題: Temporal focusing-based widefield multiphoton microscopy with spatially modulated illumination for biotissue imaging
作者: Chang, Chia-Yuan
Lin, Cheng-Han
Lin, Chun-Yu
Sie, Yong-Da
Hu, Yvonne Yuling
Tsai, Sheng-Feng
Chen, Shean-Jen
光電工程學系
Department of Photonics
關鍵字: multiphoton processes;nonlinear microscopy;three-dimensional microscopy
公開日期: 1-一月-2018
摘要: A developed temporal focusing-based multiphoton excitation microscope (TFMPEM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPEM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPEM can be condensed from 3.0 mm to 1.5 mm for a 50% improvement. By using the TFMPEM with HiLo technique as two structured illuminations at the same spatial frequency but different orientation, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression. Picture: TPEF images of the eosin-stained mouse cerebellar cortex by conventional TFMPEM (left), and the TFMPEM with HiLo technique as 1.09 mm(-1) spatially modulated illumination at 908 (center) and 08 (right) orientations. [GRAPHICS] .
URI: http://dx.doi.org/10.1002/jbio.201600287
http://hdl.handle.net/11536/144539
ISSN: 1864-063X
DOI: 10.1002/jbio.201600287
期刊: JOURNAL OF BIOPHOTONICS
Volume: 11
顯示於類別:期刊論文