標題: Bead-Based Digital Microfluidic Immunoassay for IL-1 beta Detection in Embryo Culture Medium
作者: Tseng, Hsueh-Yang
Jiang, Yi-Ting
Huang, Cheng-Yeh
Lee, Meng-Shiue
Hsu, Wensyang
Yao, Da-Jeng
機械工程學系
Department of Mechanical Engineering
關鍵字: digital microfluidic;immunoassay;embryo growth factor
公開日期: 1-Jan-2017
摘要: One of the challenges in IVF treatment is to improve the pregnancy rate of selective single embryo implantation. The concentration of IL-1 beta in embryo culture medium may help to predict pregnancy rate. Clinically, each embryo can only get small amount of sample size. However, the traditional immunoassay cannot detect biomarkers in such small sample sizes. Therefore, we combined immunoassay with the bead-based digital microfluidic chip. The chip has two advantages, low sample requirement and low detection limit. We established the mouse IL-1 beta calibration curve, confirm the Human Tubal Fluid (HTF) does not influence the test result and demonstrate that mouse IL-1 beta in clinical mouse embryo culture medium is positively correlate to cultured days. This device has a great potential for increasing IVF pregnancy rates.
URI: http://hdl.handle.net/11536/146942
期刊: 2017 IEEE 12TH INTERNATIONAL CONFERENCE ON NANO/MICRO ENGINEERED AND MOLECULAR SYSTEMS (NEMS)
起始頁: 295
結束頁: 298
Appears in Collections:Conferences Paper