標題: Nitric oxide-release study of a bio-inspired copper(i)-nitrito complex under chemical and biological conditions
作者: Chuang, Wan-Jung
Narwane, Manmath
Chen, Hsing-Yin
Kao, Chai-Lin
Huang, Bin
Hsu, Kuang-Mei
Wang, Yun-Ming
Hsu, Sodio C. N.
生物科技學系
Department of Biological Science and Technology
公開日期: 7-Oct-2018
摘要: The selective and efficient nitrite reduction process is ubiquitous in biological systems. To understand copper-mediated nitrite reduction, we developed a bio-inspired model system to investigate the mechanism of copper-containing nitrite reductase. A well-characterized copper(i)-nitrate complex with amino functionalized 2-(diphenylphosphino)aniline ligands, [(Ph2PC6H4(o-NH2))(2)Cu(ONO)], demonstrated the aniline protonation will cause NO release in an acidic environment. To further understand NO releasing ability, we also performed pH-dependency experiments and confocal imaging to release NO under physiological buffer conditions. According to titration and spectroscopic studies on the protonation reaction of complex [(Ph2PC6H4(o-NH2))(2)Cu(ONO)], we proposed a mechanistic pathway for proton transfer and NO release. Furthermore, DFT calculations predicted that the release of NO takes place via aniline in both organic and aqueous media. These results highlight the importance of the proton-rich microenvironment around the copper(i)-nitrite core to induce nitrate reduction in a chemical and biological environment.
URI: http://dx.doi.org/10.1039/c8dt02281j
http://hdl.handle.net/11536/148214
ISSN: 1477-9226
DOI: 10.1039/c8dt02281j
期刊: DALTON TRANSACTIONS
Volume: 47
起始頁: 13151
結束頁: 13157
Appears in Collections:Articles