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dc.contributor.authorMiyazaki, Junen_US
dc.contributor.authorKobayahsi, Takayoshien_US
dc.date.accessioned2020-02-02T23:54:37Z-
dc.date.available2020-02-02T23:54:37Z-
dc.date.issued2017-06-01en_US
dc.identifier.urihttp://dx.doi.org/10.3390/photonics4020032en_US
dc.identifier.urihttp://hdl.handle.net/11536/153570-
dc.description.abstractPhotothermal microscopy is useful to visualize the distribution of non-fluorescence chromoproteins in biological specimens. Here, we developed a high sensitivity and high resolution photothermal microscopy with low-cost and compact laser diodes as light sources. A new detection scheme for improving signal to noise ratio more than 4-fold is presented. It is demonstrated that spatial resolution in photothermal microscopy is up to nearly twice as high as that in the conventional widefield microscopy. Furthermore, we demonstrated the ability for distinguishing or identifying biological molecules with simultaneous muti-wavelength imaging. Simultaneous photothermal and fluorescence imaging of mouse brain tissue was conducted to visualize both neurons expressing yellow fluorescent protein and endogenous non-fluorescent chromophores.en_US
dc.language.isoen_USen_US
dc.subjectphotothermal effecten_US
dc.subjectpump-probe microscopyen_US
dc.subjectsuperresolutionen_US
dc.titlePhotothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissuesen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/photonics4020032en_US
dc.identifier.journalPHOTONICSen_US
dc.citation.volume4en_US
dc.citation.issue2en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department電子物理學系zh_TW
dc.contributor.departmentDepartment of Electrophysicsen_US
dc.identifier.wosnumberWOS:000400154700013en_US
dc.citation.woscount5en_US
Appears in Collections:Articles