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dc.contributor.authorChen, Lung-Chiehen_US
dc.contributor.authorWang, Ericken_US
dc.contributor.authorTai, Chun-Sanen_US
dc.contributor.authorChiu, Yuan-Chenen_US
dc.contributor.authorLi, Chang-Weien_US
dc.contributor.authorLin, Yan-Renen_US
dc.contributor.authorLee, Tsung-Hanen_US
dc.contributor.authorHuang, Ching-Wenen_US
dc.contributor.authorChen, Jung-Chihen_US
dc.contributor.authorChen, Wen Liangen_US
dc.date.accessioned2020-05-05T00:02:16Z-
dc.date.available2020-05-05T00:02:16Z-
dc.date.issued2020-05-01en_US
dc.identifier.issn0956-5663en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.bios.2020.112111en_US
dc.identifier.urihttp://hdl.handle.net/11536/154080-
dc.description.abstractElectrochemical biosensors possess numerous desirable qualities for target detection, such as portability and ease of use, and are often considered for point-of-care (POC) development. Label-free affinity electrochemical biosensors constructed with semiconductor manufacturing technology (SMT)-produced electrodes and a streptavidin biomediator currently display the highest reproducibility, accuracy, and stability in modern biosensors. However, such biosensors still do not meet POC guidelines regarding these three characteristics. The purpose of this research was to resolve the limitations in reproducibility and accuracy caused by problems with production of the biosensors, with the aim of developing a platform capable of producing devices that exceed POC standards. SMT production settings were optimized and bioreceptor immobilization was improved through the use of a unique linker, producing a biosensor with exceptional reproducibility, impressive accuracy, and high stability. Importantly, the three characteristics of the sensors produced using the proposed platform all meet POC standards set by the Clinical and Laboratory Standards Institute (CLSI). This suggests possible approval of the biosensors for POC development. Furthermore, the detection range of the platform was demonstrated by constructing biosensors capable of detecting common POC targets, including circulating tumor cells (CTCs), DNA/RNA, and curcumin, and the devices were optimized for POC use. Overall, the platform developed in this study shows high potential for production of POC biosensors.en_US
dc.language.isoen_USen_US
dc.subjectSemiconductor manufacturing technologyen_US
dc.subjectBiotin-streptavidin systemen_US
dc.subjectLabel-free electrochemical platformen_US
dc.subjectPoint-of-care testing (POCT)en_US
dc.titleImproving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care useen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.bios.2020.112111en_US
dc.identifier.journalBIOSENSORS & BIOELECTRONICSen_US
dc.citation.volume155en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.identifier.wosnumberWOS:000523556800017en_US
dc.citation.woscount0en_US
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