標題: Naphthalimide based smart sensor for CN-/Fe3+ and H2S. Synthesis and application in RAW264.7 cells and zebrafish imaging
作者: Naha, Sanay
Wu, Shu-Pao
Velmathi, Sivan
應用化學系
Department of Applied Chemistry
公開日期: 1-Mar-2020
摘要: Achieving selective detection of target analytes in aqueous media continues to be an arduous proposition. Herein, we report the conceptualization and synthesis of a novel tailor-fit molecular probe R based on 1,8-naphthalimide which acts as a trifunctional molecular sensor for CN-, Fe3+ and H2S. R shows colorimetric and fluorometric "on-off" relay recognition for CN- (red colour and orange emission) and Fe3+ (no colour and no emission) in 5% H2O + DMSO medium which is experimentally ascertained to be a tandem deprotonation-protonation process and is supported by H-1-NMR titration. Among all RSS (Reactive Sulphur Species), R shows selectivity for H2S through red colouration. Other coexistent anions, cations and RSS cause no discernible perturbation to the detection process. The detection of H2S is attributed to a chemodosimetric reduction of the nitro to amino group as evidenced by a potentiometric titration assay. The experimental observations are well supported by DFT theoretical calculation. The K-a for CN-/Fe3+ are 1.4 x 10(4) M-1, 6.07 x 10(4) M-1 respectively and photochemical yield of R + CN- is 0.86. Limit of detections for CN-, Fe3+ and H2S are 17.5 nM, 8.69 mu M and 8.1 mu M respectively. Receptor R is effective for real time applications, bio-compatible and has been successfully employed for confocal fluorescence imaging of RAW264.7 cell and zebrafish.
URI: http://dx.doi.org/10.1039/c9ra07998j
http://hdl.handle.net/11536/154117
DOI: 10.1039/c9ra07998j
期刊: RSC ADVANCES
Volume: 10
Issue: 15
起始頁: 8751
結束頁: 8759
Appears in Collections:Articles