標題: Low density supercritical fluids precipitation of 9-cis and all trans-beta-carotenes enriched particulates from Dunaliella salina
作者: Chen, Jian-Ren
Wu, Jia-Jiuan
Lin, Justin Chun-Te
Wang, Yuan-Chuen
Young, Chiu-Chung
Shieh, Chwen-Jen
Hsu, Shih-Lan
Chang, Cheih-Ming J.
環境工程研究所
Institute of Environmental Engineering
公開日期: 19-Jul-2013
摘要: In this study, supercritical anti-solvent (SAS) pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-beta-carotenes from Dunaliella saline. Total concentration of 9-cis (134.7 mg/g) and trans-beta-carotene (204.2 mg/g) was increased from 338.9 mg/g of the ultrasonic extract to 859.7 mg/g (338.9 for 9-cis and 520.8 for trans) of the elution fraction. The SAS pulverization of the collected fraction further produced submicron-sized particulates containing 932.1 mg/g (355.6 for 9-cis and 576.5 for trans) of total beta-carotenes with a recovery of 86.3% (83.9% for cis and 87.8% for trans). Effects of two SAS operational conditions on the purity, recovery of total beta-carotenes, mean size and morphology of the precipitates were obtained from an experimentally designed method. Generation of micronized particulates enriched with 9-cis and trans-beta-carotenes by low-density SAS was proved to be feasible and environmental benign. (C) 2013 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.chroma.2013.05.022
http://hdl.handle.net/11536/22190
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2013.05.022
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 1299
Issue: 
起始頁: 1
結束頁: 9
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