標題: A Rapid SNAP-Tag Fluorogenic Probe Based on an Environment-Sensitive Fluorophore for No-Wash Live Cell Imaging
作者: Liu, Tao-Kai
Hsieh, Pei-Ying
Zhuang, Yu-De
Hsia, Chi-Yang
Huang, Chi-Ling
Lai, Hsiu-Ping
Lin, Hung-Sheung
Chen, I-Chia
Hsu, Hsin-Yun
Tan, Kui-Thong
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 1-Oct-2014
摘要: One major limitation of labeling proteins with synthetic fluorophores is the high fluorescence background, which necessitates extensive washing steps to remove unreacted fluorophores. In this paper, we describe a novel fluorogenic probe based on an environment-sensitive fluorophore for labeling with SNAP-tag proteins. The probe exhibits dramatic fluorescence turn-on of 280-fold upon being labeled to SNAP-tag. The major advantages of our fluorogenic probe are the dramatic fluorescence turn-on, ease of synthesis, high selectivity, and rapid labeling with SNAP-tag. No-wash labeling of both intracellular and cell surface proteins was successfully achieved in living cells, and the localization of these proteins was specifically visualized.
URI: http://dx.doi.org/10.1021/cb500502n
http://hdl.handle.net/11536/25348
ISSN: 1554-8929
DOI: 10.1021/cb500502n
期刊: ACS CHEMICAL BIOLOGY
Volume: 9
Issue: 10
起始頁: 2359
結束頁: 2365
Appears in Collections:Articles