訊息分子釋放與偵測之水凝膠薄膜

Abstract

訊息分子在人體中是一種濃度變化快速之分子，扮演傳遞生物訊號之角色。本論文分成兩個部份，利用各種有機材料製作出分別能夠釋放或偵測人體中訊息分子的兩種薄膜。本論文之第一部分實現了一種以光控制一氧化氮釋放之薄膜。一氧化氮釋放薄膜是由Sodium nitroprusside dihydrate (SNP)與poly(2-hydroxyethyl methacrylate) (pHEMA)組成，SNP是一種可以釋放出一氧化氮的分子，pHEMA則是當作載體，將SNP固定在pHEMA中成膜。當不照射雷射時，一氧化氮釋放薄膜可以穩定在常溫且不釋放一氧化氮，但以雷射照射此薄膜後，SNP能夠以穩定的釋放速度(1.83x10-10莫耳/分)釋放一氧化氮。本論文之第二部分實現了一種鋅離子之偵測薄膜，該薄膜係由m-Benziporphodimethene (BPDM-H)與pHEMA組成。當BPDM-H分子被固定在pHEMA中時，BPDM-H分子的吸收譜與光激發光譜並無變化，顯示了固定在pHEMA中的BPDM-H分子仍可以如同在液體中進行鋅離子偵測。在不同鋅離子濃度的環境下時，此鋅離子偵測薄膜可以有不同的光激發光強度，其最小鋅離子濃度偵測極限為10-4莫耳濃度。

Signaling molecule is an important substance that transmits information in human body. The concentration of signaling molecule changes very fast and hence it is very interesting to produce or detect such a fast changing molecule. In this dissertation, two kinds of hydrogel films are fabricated for producing nitric oxide (NO) and detecting zinc ions. In the first part of this dissertation, an optically controlled solid-state nitric oxide donor with hydrogel matrix is realized for generating NO pulse. The solid-state NO donor is a film composed of host polymer poly(2-hydroxyethylmethacrylate) (pHEMA) and donor molecule sodium nitroprusside (SNP). The pHEMA traps the SNP tightly so the leakage of SNP or the residue of SNP is diminished. The solid-state NO donor is stable in the solution without laser excitation. After being excited by laser, the solid-state NO donor releases NO into the environment. The NO release is monitored by the NO probe 1,2-diaminoanthraquinone (DAQ) whose decrease in absorption can be observed after reacting with NO. The amount of NO that released from the solid-state NO donor after six hours laser excitation is estimated to be 1.83x10-10 mole. The second part of this dissertation demonstrates a sensing hydrogel film for zinc ion. The solid-state zinc sensing hydrogel film is a film composed of host polymer poly(2-hydroxyethylmethacrylate) (pHEMA) and probe molecule m-Benziporphodimethene (BPDM-H). The pHEMA tightly traps the BPDM-H without affecting the photoluminescence (PL) spectrum of BPDM-H. Almost no PL intensity can be observed when there is no zinc ion in the liquid environment. As zinc ion is added into the environment, the PL intensity of the zinc sensing hydrogel film increases with time. The lowest zinc concentration detection limit is 10-4 molar concentration.