表面聲波生物感測器固定化技術之研究

Abstract

表面聲波生物感測器(Surface Acoustic Wave, SAW)具有高特異性、高靈敏度及快速即時回應的感測系統裝置，其主要利用待測物的質量變化，造成壓電薄膜振盪頻率之改變來進行偵測。表面聲波生物感測器將運用於研究細菌、病毒、免疫球蛋白及DNA的量測等，且足以證明表面聲波生物感測器對於未來臨床檢驗具有相當大的潛力。 本論文研究主要將探討如何將固定蛋白質於表面聲波生物感測器之氮化鋁壓電薄膜。並透過物理性吸附法、化學共架鍵結法及表面電漿處理法等多種生物固定化技術，且進一步探討各種生物固定技術之優劣性及其可行性。 在化學共架鍵結法固定化中的C11/EDA/Proten是運用長鏈11-mercaptoundecanol 作為表面活化，再以1-ethy1-3-(3- dimethylaminopropyl) carbodiimide 作架接固定Proten 的效果較佳。 上述固定化技術都必須仰賴在壓電薄膜沉積金薄膜來進行表面活化。 為解決金薄膜對振盪頻率所造成引響，在本研究中成功開發出利用氨氣表面電漿處理法，將氮化鋁壓電薄膜活化出具有氨官能基(-NH2) 並透過架接技術將蛋白質固定於氮化鋁表面。

Surface Acoustic Wave (SAW) biosensor system has high specificity, high sensitivity and also has a real time analysis response. In this system, any mass change occurred at the piezoelectric surface after an antibody-antigen reaction is reflected either by an increase or decrease in the frequency of the piezoelectric crystal. Surface Acoustic Wave (SAW) biosensor system could be used in different clinical sample, including bacteria, virus, immunoglobulin and DNA etc. It could be proved that by clinical examination Surface Acoustic Wave (SAW) biosensor possess greater potential in the future. The purpose of this study is to find methods to immobilization protein on the Aluminum Nitride (AlN) piezoelectric surface. In this research, different physical and chemical protein immobilization methods were studied and compared with respect to their performance as well as the processes involved in the preparation. Among chemical methods, the use of 11-mercaptoundecanol for gold electrode surface activation followed by carbodiimide condensation presents the best responses when A Type Blood Cell, used as insolubilized material for A Type Blood Cell and Anti-A Type Blood Cell diction. The experimental results show that a novel method of immobilizing antibodies on Aluminum Nitride (AlN) film for Surface Acoustic Wave (SAW) was successfully developed by using amino plasma treatment.