毛細管電泳應用於藥物及食品分析之研究

Abstract

本篇博士論文中之研究使用毛細管電泳,應用於食品及藥物分析的領域,此外也將此技術結合質譜偵測法及拉曼光譜偵測法,以期建立新的分析技術.研究中配合毛細管電泳的優點,開發一些新的分析方法,以期提供一些更好的分析方法於食品及藥物的領域.食品中的重要成份包括天然成份及人工添加劑等均是研究的對象,而藥物則以類視色素這一類對人体有重要影響的化合物為研究對象,至於分析的化合物包括有類視色素,防腐劑,食用合成色素,酚酸等,分析的實際樣品涵蓋許多種類的食品及藥物.此外,對於分離的機制和實際樣品的前處理也均有探討.研究中所發展的毛細管電泳/質譜儀及毛細管電泳/拉曼光譜儀之分析系統均能成功的建立

In the thesis, capillary electrophoresis (CE) was used for food and drug analyses.Capillary electrophoresis methods and micellar electrokinetic chromatography (MEKC)methods were developed successfully for analyzing the ingredients of the foods and drugs. A MEKC method with diode-array detection was developed to separate five important retinoids. Retinoids in pharmaceutical products can be determined by combiningthe MEKC and a direct extraction method. A caillary electrophoresis method with multiwavelength deection was developed to analyze the preservatives. The preservativeswere separated within 9 min using pH 10.0 buffer modified with 2 mM alpha-cyclodextrin.The preservatives in three different foods were determined by the CE method. A CE method with diode-array detection was developed to analyze synthetic food colorants. The eight food colorants were separated within 10 min by the CE method.The colorants in different foods can be determined by the CE method without any extraction or filtration. A CE method was developed to analyze eleven phenolicacids. The phenolic acids could be separated within 13 min. The phenolic acids in fruits juices were identified and determined by the CE method. A capillary electrophoresis/continuous flow fast atom bombardment mass spectrometry (CE/CF-FABMS) system and capillary electrophoresis/Raman spectroscopy (CE/Raman) system were established in the thesis