標題: Convergent Solution Phase Synthesis of Chimeric Oligonucleotides by a 2+2 and 3+3 Phosphoramidite Strategy
作者: Chen, Chih-Hau
Chen, Wei-Yu
Chen, Yu-Chie
Lee, Ming-Juan
Huang, Chyuan-Der
Chanda, Kaushik
Sun, Chung-Ming
應用化學系
Department of Applied Chemistry
公開日期: 2010
摘要: A chimeric oligonucleotide tetramer and hexamer were synthesized by the phosphoramidite approach using a 2+2 and 3+3 strategy, respectively. The concept of convergent synthesis provides an efficient route toward the synthesis of longer chimeric oligonucleotides, such as small interfering RNA oligonucleotides without the pollution of n - 1 or shorter failures. This methodology offers an efficient and economical way to scale-up the synthesis of high purity oligonucleotides for clinical trials and commercial uses.
URI: http://hdl.handle.net/11536/6266
http://dx.doi.org/10.1071/CH09298
ISSN: 0004-9425
DOI: 10.1071/CH09298
期刊: AUSTRALIAN JOURNAL OF CHEMISTRY
Volume: 63
Issue: 2
起始頁: 227
結束頁: 235
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