完整後設資料紀錄
DC 欄位語言
dc.contributor.authorChang, Chi-Chenen_US
dc.contributor.authorHsieh, Yao-Yuanen_US
dc.contributor.authorWang, Yu-Kuoen_US
dc.contributor.authorHsu, Kung-Haoen_US
dc.contributor.authorTsai, Horng-Deren_US
dc.contributor.authorTsai, Fuu-Jenen_US
dc.contributor.authorLin, Chih-Shengen_US
dc.date.accessioned2014-12-08T15:08:04Z-
dc.date.available2014-12-08T15:08:04Z-
dc.date.issued2009-12-01en_US
dc.identifier.issn0015-0282en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.fertnstert.2008.09.007en_US
dc.identifier.urihttp://hdl.handle.net/11536/6322-
dc.description.abstractObjective: To search for novel peptides and common binding motif that specifically bind to endometriosis. Design: Prospective study. Setting: Department of Biological Science and Technology in national university. Patient(s): Specimens were divided into [1] ectopic endometrium (n = 10); [2] eutopic endometrium (n = 10). Intervention(s): Peptides specifically binding to endometriosis are screened from a phage-displaying peptide library (Ph.D.-12) by using whole-cell screening technique after an adsorption elution amplification procedure. Main Outcome Measure(s): Combinatorial peptide libraries were used to identify small molecules that bind with high affinity to receptor molecules and mimic the interaction with natural ligands. Few pans of positive phage clones with significantly positive signals were identified by ELISA and analyzed by DNA sequencing. Result(s): During the biopanning processes, the recovered phage number (10(6) pfu/mL) in parts 1, 2, 3, 4, and 5 of the study were 9, 33, 82, 142, and 169. Nine phages consistently had residue Arg, whereas six clones had a consensus motif of Arg-X-Arg-X-X-X-X-Arg. The biotin-labeled peptide bound to endometriosis cells in a dose-dependent manner, yet the control peptide revealed lesser binding activity. Conclusion(s): The novel motif is associated with higher affinity of endometriosis, which might be useful in endometriosis targeting and as potential antiendometriosis therapies. We provide one potential approach for novel therapies toward endometriosis. (Fertil Steril (R) 2009;92:1850-5. (C)2009 by American Society for Reproductive Medicine.)en_US
dc.language.isoen_USen_US
dc.subjectEndometriosisen_US
dc.subjectpeptideen_US
dc.subjectpeptide libraryen_US
dc.subjectphage displayen_US
dc.titleIdentification of novel peptides specifically binding to endometriosis by screening phage-displaying peptide librariesen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.fertnstert.2008.09.007en_US
dc.identifier.journalFERTILITY AND STERILITYen_US
dc.citation.volume92en_US
dc.citation.issue6en_US
dc.citation.spage1850en_US
dc.citation.epage1855en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000272752600008-
dc.citation.woscount1-
顯示於類別:期刊論文