開發 coralyne 及 catechol 之生物感測器

Abstract

第一部分，利用 coralyne 與腺苷具有高度專一性的結合可形成 A2-coralyne-A2 錯合物的特性，設計以三磷酸腺苷修飾金奈米粒子作為偵測 coralyne 的探針。研究指出 coralyne會抑制白血病細胞的成長，並有明顯的抗腫瘤活性，可作為癌細胞治療藥物的開發應用。實驗先以三磷酸腺苷金奈米粒子結合比色法偵測 coralyne ，得到檢量線之最低濃度為80 nM，但線性範圍狹窄。進一步應用電化學分析法偵測 coralyne ，有效改善檢量線之最低濃度下降至1 pM 且具有良好的線性範圍，並成功的應用於人體尿液的檢測。 第二部分，結合苯硼酸會與順式1,2-二醇基團的多羥基化合物進行可逆的酯化反應，以及三磷酸腺苷帶有負電荷之特性。先將金奈米粒子以電沉積方式修飾在電極上，再以硫-金鍵結將4-巰基苯硼酸修飾於金奈米粒子表面。利用鄰苯二酚與三磷酸腺苷相互競爭電極表面的4-巰基苯硼酸，進而偵測鄰苯二酚的濃度，得到檢量線之最低濃度為1 nM 且具有良號的線性範圍並實際用於環境水樣之檢測。 本實驗中，成功開發三磷酸腺苷輔助電化學的分析方法偵測抗癌藥物 coralyne 與環境汙染物鄰苯二酚，且具有良好的靈敏度與選擇性，並實際應用於真實樣品的檢測。

Adenosine was used for detecting coralyne through the coordination of A2-coralyne-A2. It has been demonstrated that coralyne can restrict the leukemia growth in cells and exhibit a noticeable anti-tumor activity, and thus providing a new medicine for the cancer therapy. We synthesized the ATP-stabilized gold nanoparticles (ATP-Au NPs). This ATP-Au NPs based colorimetric senor for coralyne detection with the sensitivity of 80 nM, but the linear range is narrow. Integration of electrochemical analysis with ATP-Au NPs for coralyne detection, the sensitivity has been effectively improved to 1 pM with a wider linear range, and the electrochemical sensor has been successfully applied to the detection in urine sample. In this study, the gold nanostructured platform was simply formed on the working electrode through controlled electrodeposition. Based on the phenylboronic acid covalently reacting with 1,2- or 1,3-diols to form five or six-membered cyclic ester, 4-MPBA was immobilized on the surface gold nanoparticles via Au-S self-assembly mechanism to capture catechol in the sample solution. Due to the highly-selective bonding of 4-MPBA with 1,2-diols, the fabricated electrode showed high selectivity for the determination of catechol with wider linear range and the sensitivity of 1 nM. Furthermore, the developed method was successfully applied to detect catechol in local river samples. The electrochemical sensors of the coralyne and catechol based on adenosine triphosphate assisted were successfully developed. Both methods have good sensitivity and selectivity, which can be applied to the detection in real samples.