渥曼青黴素降低氧化鋅奈米粒子之細胞毒性與細胞外形之影響

Abstract

奈米材料因其優越的電子、發光及催化特性而備受歡迎，但是奈米級大小的金屬、陶瓷及有機材料似乎也展現出奈米尺度特有的毒性，奈米級的粒子甚至可能污染環境或是危害人類生命。 本文藉由觀察不同尺寸氧化鋅(ZnO)奈米微粒對於小鼠胚胎纖維母細胞(NIH-3T3)細胞的死亡率與對細胞morphology影響得知氧化鋅微粒的尺寸與毒性關聯性，並希望在藉由Wortmannin作用下，能夠降低氧化鋅毒性，更進一步希望能使細胞morphology恢復正常。

Nano-sized materials are often greeted for their extraordinary electronic, light-emitting, and catalytic properties. But their unique physical characteristics raise concerns that nanometer scale of metals, ceramics, and organics could prove uniquely toxic as well. Nano-sized particles could be environmental pollution or even the loss of human life. In this study, we investigated the cytotoxicity among different size of Zinc Oxide Nanoparticles in Mouse fibroblast cell (NIH-3T3). We found that the viability and morphology of NIH-3T3 cell were affected by incorporation of ZnO nanoparticle. This effect was size and dose-dependent. Application of the cytotoxicity and morphological abnormality of NIH-3T3 cell were by means of Wortmannin.