奈米碳管電漿處理之生物固定化技術研究

Abstract

傳統奈米碳管生物固定化技術，大多都使用酸化處理奈米碳管獲得羧化；或者利用惰性金屬，例如：白金、金、銀、、、等等來修飾奈米碳管，在與生物結合。然而酸化處理會破壞奈米碳管性質，而利用惰性金屬，其價格較昂貴且不易製造。因此本論文利用電漿表面改質獲得胺(NH2）基鍵結，找出最佳電漿處理條件進行生物固定化，取代傳統方法並改善其缺點。本研究最終將找出奈米碳管生物固定化最佳條件，未來應用於奈米碳管生物感測器。 本論文實驗主要分成三個部份： 1.奈米碳管合成：利用熱分解化學氣相沈積系統(Thermal CVD)系統，以過渡金屬鎳（Ni）為觸媒製備奈米碳管，探討在前處理、碳源提供均相同條件下，改變其成長時間，其奈米碳管的成長探性與其跟時間的關連性。由分析儀器證實奈米碳管長度隨著時間增加奈米碳管長度也隨之遞增。 2.奈米碳管電漿表面改質：利用集結式電漿輔助化學氣相沉積系(PECVD)進行奈米碳管電漿表面改質，選擇氨電漿(NH3)，以不同時間進行奈米碳管表面改質，並找出最佳電漿處理奈米碳管條件。由分析儀器得知：(1)奈米碳管長度隨著電漿處理時間增加而奈米碳管長度隨之遞減；(2)在最佳電漿處理條件下，隨時間增加可提升奈米碳管品質；(3)證實經電漿處理之奈米碳管，其表面有胺(NH2）之官能基產生。 3.奈米碳管生物固定化：將最佳電漿處理奈米碳管之條件進行生物固定化處理，首先使用戊二醛(GA)交聯劑將電漿表面改質之奈米碳管進行處理後再進行生物固定化。由分析儀器證實，最佳電漿處理之奈米碳管，其表面成功固定生物抗體，並隨電漿處理時間增加而生物抗體含量隨之遞增。

In tradition methods, immobilization proteins on carbon nanotubes (CNTs), use acid treatment carbon nanotube(CNTs) to introduction carboxyl; Or utilize inertia metal, for example: Platinum, gold, silver. But acid treatment will be destroyed carbon nanotube, and utilize inertia metal, its price is more expensive and more difficult to make. So a thesis utilizes plasma treatment to modify CNTs surface to introduction amine groups (NH2). The base key is formed, use plasma treatment to introduce functional groups replace the traditional method and improve their shortcomings. Research to find out the optimum plasma treatment condition to immobilization a of proteins on carbon nanotubes (CNTs). Apply to biosensor (ex :FET-Biosensor..etc.) in the future. This thesis experiment is mainly divided into three parts: 1.CNTs synthesis Nickel catalytic-layers were deposited on silicon substrates by sputtering. Carbon nanotubes were prepared by the thermal CVD method. To demand CNTs growth under different conditions, and utilize the analytical instruments to observe changing of carbon nanotube. 2.Carbon nanotubes (CNTs) surfaces modify - Plasma treatment In this experiment, Plasma enhanced chemical vapor deposition (PECVD) system from carbon nanotubes (CNTs) by amino plasma treatment. Change of plasma treatment time, and utilize the analytical instruments to observe changing of carbon nanotube, The plasma treatment bind to amino groups incorporated into the CNTs surface. 3.Immobilization of proteins on CNTs Forced use glutaraldehyde(GA) cross-linking on the optimum plasma treatment condition of carbon nanotubes (CNTs).The experimental results show that a novel method of immobilizing mouse antibodies on CNTs was successfully developed by using amino plasma treatment.