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dc.contributor.authorLiu, Yi-Juen_US
dc.contributor.authorYao, Da-Jengen_US
dc.contributor.authorChang, Hwan-Youen_US
dc.contributor.authorLiu, Chien-Mingen_US
dc.contributor.authorChen, Chihen_US
dc.date.accessioned2014-12-08T15:10:35Z-
dc.date.available2014-12-08T15:10:35Z-
dc.date.issued2008-12-01en_US
dc.identifier.issn0956-5663en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.bios.2008.06.019en_US
dc.identifier.urihttp://hdl.handle.net/11536/8094-
dc.description.abstractThis work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis. (C) 2008 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectDNA detectionen_US
dc.subjectMagnetic beaden_US
dc.subjectQuantum dotsen_US
dc.subjectBiotin-streptavidinen_US
dc.subjectBiotinlyted linkeren_US
dc.titleMagnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7en_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.bios.2008.06.019en_US
dc.identifier.journalBIOSENSORS & BIOELECTRONICSen_US
dc.citation.volume24en_US
dc.citation.issue4en_US
dc.citation.spage558en_US
dc.citation.epage565en_US
dc.contributor.department材料科學與工程學系zh_TW
dc.contributor.departmentDepartment of Materials Science and Engineeringen_US
dc.identifier.wosnumberWOS:000261262000009-
dc.citation.woscount27-
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