標題: | Immobilization of layered double hydroxides in the fluidic system for nanoextraction of specific DNA molecules |
作者: | Chen, Jem-Kun Chan, Chia-Hao Chang, Feng-Chih 應用化學系 Department of Applied Chemistry |
公開日期: | 4-二月-2008 |
摘要: | The purpose of this study was to immobilize inorganic layered double hydroxides (LDHs) on the poly(methylmethacrylate) substrate as the media to extract the specific DNA molecules through fluidic system to enhance the efficiency of extract specific DNA molecules from extremely low concentration in sample solution. LDH immobilized through solvent swelling and plasma treatment on the polymer surface captured the specific DNA molecules lysed from Escherichia coli (E. coli) cells as the target DNA molecules with 2x10(-4) g/l of concentration in sample solution mixed biomacromolecules lysed from human blood. The encapsulated DNA molecules released through dissolving of LDHs by slight acid (pH=4-5) solution then amplified by polymerase chain reaction (PCR) process through the primers for E. coli cells. The DNA molecules amplified by PCR process were characterized by gel electrophoresis to recognize the existence of E. coli cells. The results show that immobilized LDHs could be regarded as the specific DNA detector for rapid disease diagnosis through fluidic system. (C) 2008 American Institute of Physics. |
URI: | http://dx.doi.org/10.1063/1.2840175 http://hdl.handle.net/11536/9683 |
ISSN: | 0003-6951 |
DOI: | 10.1063/1.2840175 |
期刊: | APPLIED PHYSICS LETTERS |
Volume: | 92 |
Issue: | 5 |
結束頁: | |
顯示於類別: | 期刊論文 |