標題: Solid-state sensing tip for zinc ion with double parallel optical fibers embedded in fluorescent hydrogel
作者: Su, Heng-Tsan
Tsai, May-Jywan
Chang, Gao-Fong
Hung, Chen-Hsiung
Lin, Hung-Cheng
Chou, Che-Yeu
Huang, Ding-Wen
Liang, Che-Chang
Lin, Yu-Chao
Meng, Hsin-Fei
Zan, Hsiao-Wen
Horng, Sheng-Fu
Liou, Dann-Ying
Cheng, Henrich
分子醫學與生物工程研究所
物理研究所
光電工程學系
Institute of Molecular Medicine and Bioengineering
Institute of Physics
Department of Photonics
關鍵字: Solid-state;Sensing tip;Zinc ion;Double fibers;Neuronal cultures
公開日期: 1-Nov-2015
摘要: A tip-shaped zinc ion solid-state sensor is made by two parallel optical fibers embedded closely in a sensing hydrogel film. The film is made of poly(2-hydroxyethyl methacrylate) (poly HEMA) hydrogel mixed with the selective fluorescent probe meso-2,6-Dichlorophenyltripyrrinone (TPN-Cl-2) with weight ratio of 0.025 wt%. A 405 nm laser output is sent from one fiber and the 622 nm fluorescence of the doped hydrogel is collected by the second fiber. Each fiber diameter is 370 mu m (core is 300 mu m), whose sum is roughly the tip diameter. The 0.4 cm by 0.5 cm tip has real-time response for zinc ion concentration over 10(-6) M, with marginal signal for 10(-7) M. The tip is inserted inside an oyster and successfully detects the zinc ions, showing that the sensor works in complex body fluid and tolerates certain mechanical stress. To show the potential application for medicine, the sensing film is applied for primary neuronal cultures. We report for the first time zinc ions release at concentration levels 10(-6)-10(-7) M to the medium under stress conditions of ischemia, inflammation, and intoxication. Furthermore, this correlates with the zinc levels detected by biochemical assay. Such sensing tip has great potential for biomedical monitoring ex vivo or in vivo. (C) 2015 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.orgel.2015.07.040
http://hdl.handle.net/11536/128220
ISSN: 1566-1199
DOI: 10.1016/j.orgel.2015.07.040
期刊: ORGANIC ELECTRONICS
Volume: 26
起始頁: 429
結束頁: 438
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