標題: Simultaneous detection of two growth factors from human single-embryo culture medium by a bead-based digital microfluidic chip
作者: Lee, Meng-Shiue
Hsu, Wensyang
Huang, Hong-Yuan
Tseng, Hsueh-Yang
Lee, Chia-Tung
Hsu, Chung-Yu
Shieh, Yi-Chueh
Wang, Shih-Hung
Yao, Da-Jeng
Liu, Cheng-Hsien
機械工程學系
Department of Mechanical Engineering
關鍵字: In vitro fertilization;IVF;Culture medium;Growth factor;Digital microfluidic;Immunoassay
公開日期: 15-Feb-2020
摘要: The measurement of growth factors released in a culture medium is considered to be an attractive non-invasive approach, apart from the embryo morphology, to identify the condition of an embryo development after fertilization in vitro (IVF), but the available embryo culture medium in the current method is only a few microlitres. This small sample volume, also of small concentration, makes difficult the application of a conventional detection method, such as an enzyme-linked immunosorbent assay (ELISA). A reliable detection of the growth factor from each embryo culture medium of such a small concentration hence remains a challenge. Here for the first time we report the results of measurement of not just one, but two, growth factors, human IL-1 beta and human TNF-alpha, from an individual droplet of embryo culture medium with a bead-based digital microfluidic chip. The required sample volume for a single measurement is only 520 nL; the total duration of the on-chip process is less than 40 min. Using the culture media of human embryos with normal morphologic features, we found that the concentrations of TNF-alpha change little from day 3 to day 5-6, but the concentrations of IL-1 beta for some embryos might double from day 3 to day 5-6. For other embryos even with similar normal morphologic features, some growth factors, such as IL-1 beta, might exhibit different expressions during the culture period. Those growth factors could serve to distinguish the development conditions of each embryo, not merely from an observation of embryo morphology.
URI: http://dx.doi.org/10.1016/j.bios.2019.111851
http://hdl.handle.net/11536/153772
ISSN: 0956-5663
DOI: 10.1016/j.bios.2019.111851
期刊: BIOSENSORS & BIOELECTRONICS
Volume: 150
起始頁: 0
結束頁: 0
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