標題: | Development of fibroblast-seeded collagen gels under planar biaxial mechanical constraints: a biomechanical study |
作者: | Hu, Jin-Jia Liu, Yen-Ching Chen, Guan-Wen Wang, Mei-Xuan Lee, Pei-Yuan 機械工程學系 Department of Mechanical Engineering |
關鍵字: | Fibroblast-seeded collagen gels;Tissue development;Mechanical constraints;Mechanical anisotropy;Biaxial mechanical testing;Constitutive modeling |
公開日期: | 1-Oct-2013 |
摘要: | Prior studies indicated that mechanical loading influences cell turnover and matrix remodeling in tissues, suggesting that mechanical stimuli can play an active role in engineering artificial tissues. While most tissue culture studies focus on influence of uniaxial loading or constraints, effects of multi-axial loading or constraints on tissue development are far from clear. In this study, we examined the biaxial mechanical properties of fibroblast-seeded collagen gels cultured under four different mechanical constraints for 6 days: free-floating, equibiaxial stretching (with three different stretch ratios), strip-biaxial stretching, and uniaxial stretching. Passive mechanical behavior of the cell-seeded gels was also examined after decellularization. A continuum-based two-dimensional Fung model was used to quantify the mechanical behavior of the gel. Based on the model, the value of stored strain energy and the ratio of stiffness in the stretching directions were calculated at prescribed strains for each gel, and statistical comparisons were made among the gels cultured under the various mechanical constraints. Results showed that gels cultured under the free-floating and equibiaxial stretching conditions exhibited a nearly isotropic mechanical behavior, while gels cultured under the strip-biaxial and uniaxial stretching conditions developed a significant degree of mechanical anisotropy. In particular, gels cultured under the equibiaxial stretching condition with a greater stretch ratio appeared to be stiffer than those with a smaller stretch ratio. Also, a decellularized gel was stiffer than its non-decellularized counterpart. Finally, the retained mechanical anisotropy in gels cultured under the strip-biaxial stretching and uniaxial stretching conditions after cell removal reflected an irreversible matrix remodeling. |
URI: | http://dx.doi.org/10.1007/s10237-012-0448-x http://hdl.handle.net/11536/22735 |
ISSN: | 1617-7959 |
DOI: | 10.1007/s10237-012-0448-x |
期刊: | BIOMECHANICS AND MODELING IN MECHANOBIOLOGY |
Volume: | 12 |
Issue: | 5 |
起始頁: | 849 |
結束頁: | 868 |
Appears in Collections: | Articles |
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