標題: Identification and characterization of the nuclear import and export signals of the mammalian Ste20-like protein kinase 3
作者: Lee, WS
Hsu, CY
Wang, PL
Huang, CYF
Chang, CH
Yuan, CJ
生物科技學系
Department of Biological Science and Technology
關鍵字: Mst3;Ste20;nuclear localization sequence;nuclear exporting signal;nuclear trafficking
公開日期: 13-Aug-2004
摘要: Mst3, a human Ste20-like protein kinase, has been recently demonstrated to undergo a caspase-mediated cleavage during apoptosis. The proteolytic cleavage of the C-terminus of Mst3 caused nuclear translocation of its kinase domain. This work provides evidence that Mst3 may contain a bipartite-like nuclear localization sequence (NLS) at the C-terminus of its kinase domain (residues 278-292). The removal of NLS from the kinase domain of Mst3 led to the cytoplasmic accumulation of EGFP-Mst3 (Delta277). The presence of nuclear exporting signals in the Mst3 was also demonstrated by leptomycin B-treatment and serial deletion of the C-terminal regulatory domain of Mst3. A nuclear export signal was also postulated to be in the regions of amino acids 335-386. In conclusion, Mst3 contains both NLS and NES signals, which may cooperate to control the subcellular distribution of Mst3. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.febslet.2004.07.007
http://hdl.handle.net/11536/26478
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2004.07.007
期刊: FEBS LETTERS
Volume: 572
Issue: 1-3
起始頁: 41
結束頁: 45
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