标题: B型抗谐振反射光波导表面电浆子共振生化感测器之研制与其在即时免疫分析上之应用
Fabrication of ARROW-B SPR Biosensors and Its Application to Real-Time Immunoassay
作者: 蔡承钰
Chen-Yu Tsai
黄远东
Yang-Tung Huang
电子研究所
关键字: B型抗谐振反射光波导;表面电浆子共振;即时免疫分析;ARROW-B;Surface Plasmon Resonance;Real-Time Immunoassay
公开日期: 2007
摘要: 本论文研究一种适用于水中环境的B型抗谐振反射光波导 (ARROW-B) 结构之表面电浆子共振(SPR)感测器。B型抗谐振反射光波导的入射导光区较大,能有效和单模光耦合,且可藉由调整结构来控制导光效率。使用模拟分析光场在元件中的传播特性,有系统地设计感测元件,使其在水环境下具有最佳化的感测灵敏度。在感测区前后的光场传播区设计具上下对称的光波导隔离层,使元件能稳定导光而不受外界环境影响,并于感测区附加液体流道设计,帮助生物分子附着于感测区表面进行反应。此外进行即时免疫分析上的应用,感测器针对G型老鼠免疫球蛋白分子可达约10-10 g/ml 浓度之侦测极限,并于10-10 ~ 10-7 g/ml的浓度与讯号变化具有线性关系。利用原子力显微镜可直接观察到生物分子确于金表面形成键结,最后完成抗体-抗原专一反应之检测。
In this thesis, an ARROW-B SPR sensor used in aqueous environment has been investigated. ARROW-B waveguide with a thick guiding region provides efficient coupling with a single-mode fiber, and the propagation behavior can be modulated by adjusting the structure. Modal characteristics of ARROW-B are analyzed with simulation and designed for obtaining optimum sensitivity in aqueous environment. The waveguides in front and rear of the SPR sensing region have symmetric cladding structure to improve the immunity against environmental changes, and the sensing region is configured with a liquid flow channel which assists bioagents in attaching to the gold surface for reaction. Then we submit the sensors in application to real-time immunoassay, and attain 10-10 g/ml of concentration of mouse IgG in detection limit. The power differences have a approximately linear relation with the concentration of mouse IgG between 10-10 and 10-7 g/ml. Using the Atomic Force Microscopy, the biomolecules binding to the gold surface are visualized. Finally, the specific immunoassay of antibody-antigen conjugate is demonstrated.
URI: http://140.113.39.130/cdrfb3/record/nctu/#GT009511540
http://hdl.handle.net/11536/38079
显示于类别:Thesis