標題: Identification of novel peptides specifically binding to endometriosis by screening phage-displaying peptide libraries
作者: Chang, Chi-Chen
Hsieh, Yao-Yuan
Wang, Yu-Kuo
Hsu, Kung-Hao
Tsai, Horng-Der
Tsai, Fuu-Jen
Lin, Chih-Sheng
生物科技學系
Department of Biological Science and Technology
關鍵字: Endometriosis;peptide;peptide library;phage display
公開日期: 1-Dec-2009
摘要: Objective: To search for novel peptides and common binding motif that specifically bind to endometriosis. Design: Prospective study. Setting: Department of Biological Science and Technology in national university. Patient(s): Specimens were divided into [1] ectopic endometrium (n = 10); [2] eutopic endometrium (n = 10). Intervention(s): Peptides specifically binding to endometriosis are screened from a phage-displaying peptide library (Ph.D.-12) by using whole-cell screening technique after an adsorption elution amplification procedure. Main Outcome Measure(s): Combinatorial peptide libraries were used to identify small molecules that bind with high affinity to receptor molecules and mimic the interaction with natural ligands. Few pans of positive phage clones with significantly positive signals were identified by ELISA and analyzed by DNA sequencing. Result(s): During the biopanning processes, the recovered phage number (10(6) pfu/mL) in parts 1, 2, 3, 4, and 5 of the study were 9, 33, 82, 142, and 169. Nine phages consistently had residue Arg, whereas six clones had a consensus motif of Arg-X-Arg-X-X-X-X-Arg. The biotin-labeled peptide bound to endometriosis cells in a dose-dependent manner, yet the control peptide revealed lesser binding activity. Conclusion(s): The novel motif is associated with higher affinity of endometriosis, which might be useful in endometriosis targeting and as potential antiendometriosis therapies. We provide one potential approach for novel therapies toward endometriosis. (Fertil Steril (R) 2009;92:1850-5. (C)2009 by American Society for Reproductive Medicine.)
URI: http://dx.doi.org/10.1016/j.fertnstert.2008.09.007
http://hdl.handle.net/11536/6322
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2008.09.007
期刊: FERTILITY AND STERILITY
Volume: 92
Issue: 6
起始頁: 1850
結束頁: 1855
Appears in Collections:Articles