應用於細胞圖形化和成長控制之磁場平台設計與製造

Abstract

本論文主要是設計及製造一個可應用於細胞圖形化和成長控制之磁場平台。在矽基材上製造5.5圈的螺旋電感，通上60Hz的AC電流，可以產生mT 等級的區域性磁場。磁場的強度及分布，還有可能的焦耳熱效應皆加以計算、模擬及量測。當加上熱電致冷片時，可以有效消除升溫，並將整個平台維持在攝氏37度。根據磁場可以增加細胞效應的研究[14-17]，加上細胞成長速率的增加和磁場強度成正比的關係，我們相信將細胞置於平台上時，電感的不同區域產生的不同的磁場強度會導致細胞有不同的成長效率，因而產生細胞圖形化的結果。

This thesis presents a magnetic microplatform design using localized magnetic field for cell patterning and growth controlling application. A 5.5-turns spiral inductor is designed and fabricated on a silicon substrate to generate the localized field with a mT-order magnitude while being loaded with 60Hz, >0.1A, AC current source. The magnetic field strength, field distribution on the spiral inductor is calculated, simulated using Ansoft Maxewell and measured. The possible joule heating effect is calculated, simulated using Ansoft-ePhysics and measured as well. In order to avoid temperature rise, a thermoelectric cooler is attached at the bottom of the device to control the whole platform at 37C. The magnetic field distribution of the inductor could make cell proliferation rate difference on cells different positions so cell patterning could be realized.