標題: Overcoming Multidrug Resistance of Breast Cancer Cells by the Micellar Doxorubicin Nanoparticles of mPEG-PCL-Graft-Cellulose
作者: Chen, Chao-Hsuan
Cuong, Nguyen-Van
Chen, Yung-Tsung
So, Regina Cheng
Liau, Ian
Hsieh, Ming-Fa
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Methoxy-Poly(ethylene glycol);Poly(epsilon-caprolactone);Multidrug Resistance;P-Glycoprotein;Human Breast Cancer Cells
公開日期: 1-Jan-2011
摘要: The amphiphilic block copolymer methoxy-poly(ethylene glycol)-poly(epsilon-caprolactone) (mPEG-PCL) was grafted to 2-hydroxyethyl cellulose (HEC) to produce nano-sized micellar nanoparticles. The nanoparticles were loaded with anti-tumor drug, doxorubicin (DOX) and the size of the DOX-loaded nanoparticles were determined by dynamic light scattering (DLS) in aqueous solution to be from 197.4 to 230 nm. The nanoparticles subjected to co-culture with macrophage cells showed that these nanoparticles used as drug carrier are not recognized as foreign bodies. Overexpression of P-glycoprotein (P-gp) is an important factor in the development of multidrug resistance (MDR) in many cancer cells. In this study, Western blot and Rhodamine 123 were used to monitor the relative P-glycoprotein expression in human breast cancer cell lines MCF-7/WT and MCF-7/ADR. The endocytosis of the DOX-loaded nanoparticles by breast cancer cells is more efficient observed under a confocal laser scanning microscopy (CLSM) and a flow cytometry in MCF7/ADR cells, compared to the diffusion of the free drug into the cytoplasm of cells. Based on these findings, we concluded that the nanoparticles made from mPEG-PCL-g-cellulose were effective in overcoming P-gp efflux in MDR breast cancer cells.
URI: http://dx.doi.org/10.1166/jnn.2011.3102
http://hdl.handle.net/11536/26123
ISSN: 1533-4880
DOI: 10.1166/jnn.2011.3102
期刊: JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume: 11
Issue: 1
起始頁: 53
結束頁: 60
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