標題: | Overcoming Multidrug Resistance of Breast Cancer Cells by the Micellar Doxorubicin Nanoparticles of mPEG-PCL-Graft-Cellulose |
作者: | Chen, Chao-Hsuan Cuong, Nguyen-Van Chen, Yung-Tsung So, Regina Cheng Liau, Ian Hsieh, Ming-Fa 應用化學系 應用化學系分子科學碩博班 Department of Applied Chemistry Institute of Molecular science |
關鍵字: | Methoxy-Poly(ethylene glycol);Poly(epsilon-caprolactone);Multidrug Resistance;P-Glycoprotein;Human Breast Cancer Cells |
公開日期: | 1-一月-2011 |
摘要: | The amphiphilic block copolymer methoxy-poly(ethylene glycol)-poly(epsilon-caprolactone) (mPEG-PCL) was grafted to 2-hydroxyethyl cellulose (HEC) to produce nano-sized micellar nanoparticles. The nanoparticles were loaded with anti-tumor drug, doxorubicin (DOX) and the size of the DOX-loaded nanoparticles were determined by dynamic light scattering (DLS) in aqueous solution to be from 197.4 to 230 nm. The nanoparticles subjected to co-culture with macrophage cells showed that these nanoparticles used as drug carrier are not recognized as foreign bodies. Overexpression of P-glycoprotein (P-gp) is an important factor in the development of multidrug resistance (MDR) in many cancer cells. In this study, Western blot and Rhodamine 123 were used to monitor the relative P-glycoprotein expression in human breast cancer cell lines MCF-7/WT and MCF-7/ADR. The endocytosis of the DOX-loaded nanoparticles by breast cancer cells is more efficient observed under a confocal laser scanning microscopy (CLSM) and a flow cytometry in MCF7/ADR cells, compared to the diffusion of the free drug into the cytoplasm of cells. Based on these findings, we concluded that the nanoparticles made from mPEG-PCL-g-cellulose were effective in overcoming P-gp efflux in MDR breast cancer cells. |
URI: | http://dx.doi.org/10.1166/jnn.2011.3102 http://hdl.handle.net/11536/26123 |
ISSN: | 1533-4880 |
DOI: | 10.1166/jnn.2011.3102 |
期刊: | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY |
Volume: | 11 |
Issue: | 1 |
起始頁: | 53 |
結束頁: | 60 |
顯示於類別: | 期刊論文 |