即時感測一氧化氮之水凝膠薄膜

Abstract

一氧化氮在生物體內的循環生理、免疫殺菌及行為記憶各方面扮演著重要的角色。然而，由於一氧化氮在生理環境中的生命期相當的短，大約為5秒，因此發展具有即時偵測一氧化氮濃度變化之感測器是相當重要的。 在此，我們發展出一種由2-Hydroxyethyl methacrylate（HEMA）所組成水凝膠薄膜，其具有的高透水性、高透明度與可撓性非常適合於生物感測的實驗所使用。 我們分別將螢光型的NO probe: 11,16-bisphenyl-6,6,21,21 - tetramethyl-m-benzi - 6,21 - porphodimethenochloro-zinc(II)（BPDM–H–ZnCl，ZnC1）與1,2-Diaminoanthraquinone（DAQ）摻入HEMA製作出一氧化氮的感測薄膜。以ZnC1的感測薄膜來說，此薄膜能於水中與HBSS中讀到NO的訊號，並且能利用靜電紡絲的技術提升薄膜在水中的感測極限，但也發現ZnC1感測NO的機制是由pH的變化所造成。在另一方面，DAQ的感應薄膜亦能讀取NO於buffer中的變化，也能排除由pH下降所造成的影響，故DAQ感應薄膜會是未來發展的重要方向。

Nitric oxide (NO) plays important role in human body. NO produced endogenously by one cell can transmit through cell membranes and regulates the function of another cell. NO relaxes the smooth muscle in the walls of the arterioles, regulates the blood pressure, and inhibits the aggregation of platelets. However, the lifetime of NO is short around 5 seconds in physiological environment. It is therefore crucial to develop a real-time NO sensor. In this work, a sensing hydrogel film consists of 2-Hydroxyethyl methacrylate（HEMA）is developed. The film is which is water permeable,transparency,flexible, and suitable for biological experiment. A fluorescent probe 11,16-bisphenyl-6,6,21,21 - tetramethyl-m-benzi - 6,21 - porphodimethenochloro-zinc(II) （BPDM–H–ZnCl） or 1,2-Diaminoanthraquinone（DAQ） can be blended with HEMA to form the NO sensing hydrogel film respectively. For the ZnC1 sensing film, it is demonstrated that the PL decreases as NO exist in water and HBSS. Increasing the surface area by electrospinning can increase the NO concentration detection limit. But we found the decrease in PL comes from the change in pH value. As for the DAQ sensing film, it is also demonstrated that it can detect NO in buffer. pH value of the environment will not cause decrease in absorption peak. In the future, development in DAQ sensing film is important.