標題: | Cloning and characterization of monacolin K biosynthetic gene cluster from Monascus pilosus |
作者: | Chen, Yi-Pei Tseng, Ching-Ping Liaw, Li-Ling Wang, Chun-Lin Chen, I-Ching Wu, Wen-Jung Wu, Ming-Der Yuan, Gwo-Fang 生物科技學系 Department of Biological Science and Technology |
關鍵字: | monacolin K;polyketide synthases;Monascus pilosus;bacterial artificial chromosome |
公開日期: | 23-Jul-2008 |
摘要: | Monacolin K is a secondary metabolite synthesized by polyketide synthases (PKS) from Monascus, and it has the same structure as lovastatin, which is mainly produced by Aspergillus terreus. In the present study, a bacterial artificial chromosome (BAC) clone, mps01, was screened from the BAC library constructed from Monascus pilosus BCRC38072 genomic DNA. The putative monacolin K biosynthetic gene cluster was found within a 42 kb region in the mps01 clone. The deduced amino acid sequences encoded by the nine genes designated as mokA-mokl, which share over 54% similarity with the lovastatin biosynthetic gene cluster in A. terreus, were assumed to be involved in monacolin K biosynthesis. A gene disruption construct designed to replace the central part of mokA, a polyketide synthase gene, in wild-type M. pilosus BCRC38072 with a hygromycin B resistance gene through homologous recombination, resulted in a mokA-disrupted strain. The disruptant did not produce monacolin K, indicating that mokA encoded the PKS responsible for monacolin K biosynthesis in M. pilosus BCRC38072. |
URI: | http://dx.doi.org/10.1021/jf800595k http://hdl.handle.net/11536/8558 |
ISSN: | 0021-8561 |
DOI: | 10.1021/jf800595k |
期刊: | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY |
Volume: | 56 |
Issue: | 14 |
起始頁: | 5639 |
結束頁: | 5646 |
Appears in Collections: | Articles |
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